Activating Compound | Comment | Organism | Structure |
---|---|---|---|
H2O2 | oxidative stress activates OMTK1 | Medicago sativa | |
additional information | MG115 induces OMTK1 | Medicago sativa | |
NaCl | - |
Medicago sativa |
Cloned (Comment) | Organism |
---|---|
OMTK1, DNA and amino acid sequence determination, expression as GST-fusion protein, transient co-expression of Myc-tagged OMTK1 and HA-tagged MMK3 in Arabidopsis thaliana protoplasts, in vitro trabscription and translation of wild-type and mutant OMTK1 by reticulocyte lysate | Medicago sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an OMTK1 mutant reduced in MMK3 complex formation shows decreased MMK3 and cell death activation | Medicago sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
protoplast | basal activity level of OMTK1 | Medicago sativa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK3 | Medicago sativa | a MAPKK, OMTK1 channels oxidative stress signaling through direct interaction with MAPK MMK3 increasing the cell death rate, OMTK1 and MMK3 form a complex in vivo | ADP + phosphorylated MKK3 | - |
? | |
additional information | Medicago sativa | the OMTK1 kinase domain is involved in activation of signaling pathways in case of oxidative stress to induce cell death playing a MAPK scaffolding role, OMTK1 shows low constitutive activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | Q7XTK4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK3 | a MAPKK | Medicago sativa | ADP + phosphorylated MKK3 | - |
? | |
ATP + MKK3 | a MAPKK, OMTK1 channels oxidative stress signaling through direct interaction with MAPK MMK3 increasing the cell death rate, OMTK1 and MMK3 form a complex in vivo | Medicago sativa | ADP + phosphorylated MKK3 | - |
? | |
additional information | the OMTK1 kinase domain is involved in activation of signaling pathways in case of oxidative stress to induce cell death playing a MAPK scaffolding role, OMTK1 shows low constitutive activity | Medicago sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAPKKK | - |
Medicago sativa |
MEKK1 | - |
Medicago sativa |
OMTK1 | - |
Medicago sativa |
oxidative stress-activated MAP triple-kinase 1 | - |
Medicago sativa |
putative mitogen-activated protein kinase 1 | - |
Medicago sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Medicago sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Medicago sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Medicago sativa |